PC213L
Anti-GABA Rabbit pAb
lyophilized, Calbiochem®
Synonyme(s) :
Anti-Gamma Amino-Butyric Acid
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized
Ne contient pas
preservative
Espèces réactives
rat
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Isotype
IgG
Conditions d'expédition
ambient
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Gabra1(29705)
Description générale
Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.
Immunogène
GABA
Rat
Application
Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
Undiluted serum.
Reconstitution
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Remarque sur l'analyse
Positive Control
Rat thalamus or cerebellum
Rat thalamus or cerebellum
Autres remarques
Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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