NE1015
Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)
liquid, clone SMI-22, Calbiochem®
Synonyme(s) :
Anti-GFAP Cocktail
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
SMI-22, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
human, bovine, mouse, guinea pig, porcine, sheep, canine, rat, chicken
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG2b
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GFAP(2670)
Description générale
Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
Immunogène
Bovine
purified bovine GFAP protein
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
Undiluted ascites.
Reconstitution
Upon initial thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
Astrocytes or cytoskeletal preparations
Astrocytes or cytoskeletal preparations
Autres remarques
This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
Vick, W.W., et al. 1987. Acta. Cytol.31, 816.
McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.
Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.
McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.
Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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