MABS1280M

Anti-mS2i6A Antibody, clone CL-6

clone CL-6, from mouse

• Synonyme(s) : N6-isopenetyl-2-thiomethyladenosine
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: CL-6, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): mammals
• Conditionnement: antibody small pack of 25 μg
• Technique(s): ELISA: suitable; dot blot: suitable
• Isotype: IgG2bκ
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

The mS2i6A is an evolutionally conserved modification found in the tRNA. The hypermodified mS2i6A at position 37 occurs frequently in tRNAs that read codons starting with uridine. The mS2 group of mS2i6A is critical for the accurate decoding of Tyr and Phe codons. It has been shown that a deficiency of mS2i6A in the cognate poly(U) reader tRNA(Phe) leads to increased misreading when the near-cognate competitor tRNA4(Leu) is wild-type. It can lead to higher rejection rate of tRNA4(Leu) by more aggressive proofreading on the wild-type ribosome. It is believed that Cdk5rap1 specifically catalyzes the mS2 modification of mt-tRNAs and contributes to mitochondrial function in vivo. This modification regulates efficient mitochondrial translation and energy metabolism in mammals. Cdk5rap1 knockout mice that are deficient in the 2-methylthio modification in mt-tRNAs bearing Ser(UCN), Phe, Tyr and Trp, impaired mitochondrial protein synthesis is observed, which causes respiratory defects of mitochondria and myopathy in vivo. (Ref.: Deuchler, M et al. (2016). Cell Mol Life Sci. 73 (16); 3075-3095; Fakruddin, M., et al. (2017). Nucleic Acids Res. 2017 Nov 16;45(20):11954-11961).

Spécificité

Clone CL-6 recognizes wild-type total RNA, but not Cdk5rap1 KO total RNA.

Immunogène

A synthesized mS2i6A nucleotide conjugated to BSA.

Application

Anti-mS2i6A, clone CL-6, Cat. No. MABS1280, is a mouse monoclonal antibody that detects mS2i6A and has been tested for use in Dot Blot and ELISA.

Dot Blot Analysis: A 1:200 dilution of this antibody detected mS2i6A in Mouse liver total RNA and Cdk5rap1 KO mouse total RNA (Courtesy of Fan-Yan Wei, Ph.D., Faculty of Life Sciences, Kumamoto University Japan).

ELISA Analysis: A representative lot detected mS2i6A in ms2i6A and i6A wells (Courtesy of Fan-Yan Wei, Ph.D., Faculty of Life Sciences, Kumamoto University Japan).

Research Category
Signaling

Qualité

Evaluated by Dot Blot in Mouse Liver Total RNA.

Dot Blot Analysis: A 1:2,000 dilution of this antibody detected mS2i6A in Mouse Liver total RNA.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable