MAB367
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
clone M2-2-B3, Chemicon®, from rat
Synonyme(s) :
Muscarinic Receptor Antibody
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rat
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
M2-2-B3, monoclonal
Espèces réactives
human, monkey, rat
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotype
IgG2a
Adéquation
not suitable for immunohistochemistry (Paraffin)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CHRM2(1129)
Spécificité
m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.
SPECIES REACTIVITIES:
Expected to react with most mammalian species.
SPECIES REACTIVITIES:
Expected to react with most mammalian species.
Immunogène
i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
Application
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.
Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells
Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Forme physique
Format: Purified
Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jay F Muller et al.
The Journal of comparative neurology, 524(12), 2400-2417 (2016-01-19)
Activation of M2 muscarinic receptors (M2Rs) in the rat anterior basolateral nucleus (BLa) is critical for the consolidation of memories of emotionally arousing events. The present investigation used immunocytochemistry at the electron microscopic level to determine which structures in the
Lisa Lambert et al.
Frontiers in cellular neuroscience, 12, 450-450 (2018-12-18)
Our aim was to examine the dynamics of the muscarinic m2 receptor (m2R), a G-protein coupled receptor (GPCR), after agonist activation in living hippocampal neurons, and especially clathrin dependency endocytosis. We have previously shown that the m2R undergoes agonist-induced internalization
Roles of M2 and M4 muscarinic receptors in regulating acetylcholine release from myenteric neurons of mouse ileum.
Takeuchi, T; Fujinami, K; Goto, H; Fujita, A; Taketo, MM; Manabe, T; Matsui, M; Hata, F
Journal of Neurophysiology null
Maria Medalla et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(44), 15611-15625 (2012-11-02)
The anterior cingulate cortex (ACC) and dorsolateral prefrontal cortices (DLPFC) share robust excitatory connections. However, during rapid eye movement (REM) sleep, when cortical activity is dominated by acetylcholine, the ACC is activated but DLPFC is suppressed. Using pathway tracing and
Răzvan Gămănuţ et al.
Neuron, 97(3), 698-715 (2018-02-09)
The inter-areal wiring pattern of the mouse cerebral cortex was analyzed in relation to a refined parcellation of cortical areas. Twenty-seven retrograde tracer injections were made in 19 areas of a 47-area parcellation of the mouse neocortex. Flat mounts of
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