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Merck
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Key Documents

MAB316

Sigma-Aldrich

Anti-GABA Antibody, clone 5A9

clone 5A9, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

5A9, monoclonal

Réactivité de l'espèce (prédite par homologie)

mammals

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GABRA1(2554)

Spécificité

Specific for GABA and does not cross-react with other amino acids.

Immunogène

GABA-coupled to BSA with glutaraldehyde.

Application

Anti-GABA Antibody, clone 5A9 detects level of GABA & has been published & validated for use in ELISA, IH & WB.
Immunohistochemistry on frozen sections if perfusion fixed. Glutaraldehyde must be included in fixative with paraffin sections: 1:200-1:400. Typical fixative might be 4% PFA, 1% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.3 .

Immunoblotting not recommended due to low molecular wgt: 103Da.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Forme physique

Format: Purified
Protein A purified
Purified mouse monoclonal in PBS, pH 7.4, 1% BSA, and 0.1% sodium azide as a preservative.

Stockage et stabilité

Maintain refrigerated at 2–8°C for up to 6 months.

Remarque sur l'analyse

Control
Myeloblasts, lymphocytes, erythroblasts

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Alain C Burette et al.
The Journal of comparative neurology, 522(9), 2164-2178 (2014-03-19)
The 53 kDa insulin receptor substrate protein (IRSp53) is highly enriched in the brain. Despite evidence that links mutations of IRSp53 with autism and other neuropsychiatric problems, the functional significance of this protein remains unclear. We used light and electron
Shogo Soma et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(3), 485-502 (2018-11-28)
It is well known that the posterior parietal cortex (PPC) and frontal motor cortices in primates preferentially control voluntary movements of contralateral limbs. The PPC of rats has been defined based on patterns of thalamic and cortical connectivity. The anatomical
Masakazu Uematsu et al.
Cerebral cortex (New York, N.Y. : 1991), 18(2), 315-330 (2007-05-23)
Although neocortical GABAergic (gamma-aminobutyric acidergic) interneurons have been the focus of intense study, especially in the rat, a consensus view of the functional diversity and organization of inhibitory cortical neurons has not yet been achieved. To better analyze GABAergic neurons
Neelima Bhargava et al.
In vitro cellular & developmental biology. Animal, 46(8), 685-692 (2010-07-16)
A very small population of choline acetyltransferase (ChAT) immunoreactive cells is observed in all layers of the adult hippocampus. This is the intrinsic source of the hippocampal cholinergic innervation, in addition to the well-established septo-hippocampal cholinergic projection. This study aimed
Naomi Karoubi et al.
Frontiers in neuroanatomy, 10, 106-106 (2016-11-29)
Over recent years, the seven-spot archerfish (Toxotes chatareus) has emerged as a new model for studies in visual and behavioral neuroscience thanks to its unique hunting strategy. Its natural ability to spit at insects outside of water can be used
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