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MAB1998

Sigma-Aldrich

Anti-Integrin α2β1 Antibody, clone BHA2.1

clone BHA2.1, Chemicon®, from mouse

Synonyme(s) :

VLA-2

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

BHA2.1, monoclonal

Espèces réactives

human, canine, pig

Fabricant/nom de marque

Chemicon®

Technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotype

IgG1κ

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ITGA2(3673)

Spécificité

Reacts with the collagen receptor VLA-2 (alpha-2beta-1) integrin complex.

Application

Anti-Integrin α2β1 Antibody, clone BHA2.1 is an antibody against Integrin α2β1 for use in FC, IH, IH(P) & IP.
Research Category
Cell Structure
Research Sub Category
Integrins
flow cytometry: 10 μg/mL

Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1

immunohistochemistry in frozen tissue sections: 5 μg/mL

Immunohistochemistry in paraffin sections: 1:50. Block endogenous peroxidase using 0.5% H202 in 80% ethanol, 30 min and unspecific reactions using NGS 1:5 in PBS, 20min RT. Bouin′s fixative solution has been used successfully. Incubation time, conditions: 24 h; 4°C; pretreatment: 0.1% trypsin, 30 min, 37°C. Intracytoplasmic staining near cell membranes.

Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.

Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.

Final working dilutions must be determined by end user.

Forme physique

Format: Purified
Protein A purified
Purified immunoglobulin from Protein A chromatography. Presented in liquid in 0.02M PB pH 7.6, 0.25M NaCl, 0.1% sodium azide.

Stockage et stabilité

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Skin, CD4 & CD8 positive T cells

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Amna Abderrazak et al.
Frontiers in immunology, 9, 2269-2269 (2018-10-31)
β1 integrins are critical for T cell migration, survival and costimulation. The integrin α2β1, which is a receptor for collagen, also named VLA-2, is a major costimulatory pathway of effector T cells and has been implicated in arthritis pathogenesis. Herein
Mehdi Dehghani et al.
International journal of cell biology, 2014, 981750-981750 (2015-01-13)
The mechanisms by which prostate cancer (PCa) cell adhesion and migration are controlled during metastasis are not well understood. Here, we studied the effect of CXCL12 in PCa cell adhesion and spreading in DU145 and PC3 cell lines using as
Feng Luo et al.
International journal of nanomedicine, 13, 7657-7667 (2018-12-13)
It is well known that zirconia materials have good biocompatibility; however, little is known regarding the mechanism by which cells attach to these materials. The purpose of this study is to elucidate the mechanism of cell attachment. In this study
Xiaodong Feng et al.
International journal of cell biology, 2013, 231279-231279 (2013-06-06)
Angiogenesis is a highly regulated event involving complex, dynamic interactions between microvascular endothelial cells and extracellular matrix (ECM) proteins. Alteration of ECM composition and architecture is a hallmark feature of wound clot and tumor stroma. We previously reported that during
Chemically programmed antibodies targeting multiple alpha(v) integrins and their effects on tumor-related functions in vitro.
Goswami, RK; Bajjuri, KM; Forsyth, JS; Das, S; Hassenpflug, W; Huang, ZZ; Lerner et al.
Bioconjugate Chemistry null

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