MAB1254

Anti-Chymase Antibody, clone B7

clone B7, 2.4 mg/mL, Chemicon^®

• Synonyme(s) : Mast Cell Protease I
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: B7, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Concentration: 2.4 mg/mL
• Technique(s): dot blot: suitable; immunohistochemistry: suitable
• Isotype: IgG1κ
• Adéquation: not suitable for Western blot
• Numéro d'accès NCBI: NM_001836.2
• Numéro d'accès UniProt: P23946
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CMA1(1215)

Description

Description générale

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Spécificité

Reacts with human mast cell chymase.

Immunogène

Chymase purified from human skin.

Application

Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.

Dot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Forme physique

Format: Purified

Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.

Remarque sur l'analyse

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable