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Key Documents

HTS142RTA

Sigma-Aldrich

Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells

Human

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About This Item

Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.84

product name

Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells, Human EP4 GPCR frozen cells for Calcium Flux FLIPR Assays.

Source biologique

human

Niveau de qualité

100

Fabricant/nom de marque

Ready-to-Assay

Technique(s)

calcium flux assay: suitable

Numéro d'accès NCBI

NM_000958

Méthode de détection

fluorometric

Conditions d'expédition

dry ice

Description générale

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and further modified by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGE2 causes pain, vasodilation, immunosuppression of T cells, bone remodeling and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. EP4 couples primarily to Gs to increase intracellular cAMP levels. During neonatal development, EP4 participates in closure of the ductus arteriosus, a process required for switching circulation from the placenta to the lungs (Nguyen et al., 1997). In addition, EP4 mediates PGE2-induced bone formation by promoting osteoblastogenesis, and selective EP4 agonists are being evaluated as potential treatments for osteoporosis (Yoshida et al., 2002). Millipore’s cloned human EP4-expressing cells are made in the Chem-1 host, which supports high levels of recombinant EP4 expression on the cell surface and contains high levels of the promiscuous G protein G15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at EP4.

Description de la lignée cellulaire

GPCR Cell Lines
Host cells: Chem-1

Application

Human EP4 GPCR frozen cells for Calcium Flux FLIPR Assays.

Actions biochimiques/physiologiques

GPCR Class: A
Protein Target: EP4
Target Sub-Family: Prostanoid

Composants

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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