ABE1318

Anti-SMARCD2 (BAF60B)

from rabbit, purified by affinity chromatography

• Synonyme(s) : SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B, BAF60B
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_001091896.1
• Numéro d'accès UniProt: Q92925
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SMARCD2(6603)

Description

Description générale

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (UniProt: Q92925; also known as 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B, BAF60B) is encoded by the SMARCD2 (also known as BAF60B, PRO2451) gene (Gene ID: 6603) in human. It is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and regulate transcription of certain genes by altering the chromatin structure around those genes. Three members of BAF60 family have been described and are designated as FAF60 A, B, and C. BAF60B is expressed in immune cells, muscle, and pancreas tissues. Two isoforms of BAF60B have been described that are produced by alternative splicing. The SWIB domain of BAF60B is localized in amino acids 307-382. BAF60B is reported to interact with multiple transcription factors and may bridge transcription factors with SWI/SNF complexes. At least 11 subunits of SWI/SNF complex have been discovered, including 2 enzymatic ATPase subunits (Brm and Brg1) and 10 Brg1/Brm associated factors (BAFs), which are named according to their molecular weights. BAF60B is shown to link chromatin opening to ATM activation by facilitating ATM recruitment to the open chromatin regions of a panel of hepatic gene loci. It is required for 3TF (transcription factors Foxa3, Hnf1 and Gata4) - induced activation of ATM and facilitates the recruitment of ATM to the SWI/ SNF complex. (Ref.: Ji, S., et al. (2017). Cell Res. 27(5); 642-656; Lores, P., et al. (2010). FEBS. J. 277(6); 1453-1464).

Spécificité

This rabbit polyclonal antibody detects SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (SMARCD2) in human cells. It targets an epitope within 13 amino acids from the N-terminal region.

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (BAF60B) protein.

Application

Anti-SMARCD2 (BAF60B), Cat. No. ABE1318, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotti

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SMARCD2 (BAF60B) in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SMARCD2 (BAF60B) in HeLa, A431, HUVEC and NIH/3T3 cell lines.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SMARCD2 (BAF60B) in human cerebral cortex, human testis, and human ovarian cancer tissue sections.

Qualité

Evaluated by Western Blotting in Jurkat nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SMARCD2 (BAF60B) in 10 µg of Jurkat cell nuclear extract.

Description de la cible

~59 kDa observed; 58.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1