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Key Documents

482728

Sigma-Aldrich

Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-iNOS

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

mouse, rat, human

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NOS1(4842)

Description générale

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
Recognizes the ~130 kDa iNOS protein.
This Anti-Nitric Oxide Synthase, Inducible Rabbit pAb is validated for use in Frozen Sections, WB, ICC, Paraffin Sections for the detection of Nitric Oxide Synthase, Inducible.

Immunogène

Mouse
a synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH

Application

Frozen Sections (1:500)

Immunoblotting (1:2000)

Immunocytochemistry (1:500)

Paraffin Sections (1:500)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
Raw 264.7 cells treated with IFNγ and LPS

Autres remarques

Exhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.



Recommended Procedure for Immunocytochemistry of Anti-iNOS:

1. Grow cells in appropriate medium.

2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.

3. Wash cells 3-5 min with phosphate buffered saline (PBS).

4. Permeabilize with 2% saponin for 10 min at room temperature.

5. Fix in 4% formalin for 10 min at room temperature.

6. Wash 3-5 min with PBS.

7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.

8. Wash 3-5 min with PBS.

9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).

10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


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Consulter la Bibliothèque de documents

Jae Hwan Kim et al.
Experimental neurobiology, 26(6), 380-389 (2018-01-06)
Ischemic preconditioning (IP) is one of the most important endogenous mechanisms that protect the cells against ischemia-reperfusion (I/R) injury. However, the exact molecular mechanisms remain unclear. In this study, we showed that changes in the level of agmatine were correlated
Dejia Li et al.
PloS one, 6(6), e21618-e21618 (2011-07-09)
Nitrosative stress compromises force generation in Duchenne muscular dystrophy (DMD). Both inducible nitric oxide synthase (iNOS) and delocalized neuronal NOS (nNOS) have been implicated. We recently demonstrated that genetic elimination of nNOS significantly enhanced specific muscle forces of the extensor
David L Schreibman et al.
Neurocritical care, 29(2), 253-263 (2018-04-28)
Spontaneous intracerebral hemorrhage (ICH) leaves most survivors dependent at follow-up. The importance of promoting M2-like microglial responses is increasingly recognized as a key element to ameliorate brain injury following ICH. The osmotherapeutic agents, mannitol and hypertonic saline (HTS), which are
S Nuedling et al.
FEBS letters, 502(3), 103-108 (2001-10-05)
Physiological effects of estrogen on myocardium are mediated by two intracellular estrogen receptors, ERalpha and ERbeta, that regulate transcription of target genes through binding to specific DNA target sequences. To define the role of ERbeta in the transcriptional activation of
Chanyong Yun et al.
Mediators of inflammation, 2016, 8027537-8027537 (2016-09-21)
The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and

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