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Key Documents

475989

Sigma-Aldrich

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

Synonyme(s) :

MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue

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About This Item

Formule empirique (notation de Hill):
C18H16BrN5S
Numéro CAS:
298-93-1
Poids moléculaire :
414.32
Numéro MDL:
MFCD00011964
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.25

Niveau de qualité

100

Pureté

≥98% (silver nitrate titration)

Forme

solid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Impuretés

≤0.5% Sulfated ash

Couleur

yellow

Solubilité

PBS: 2 mg/mL

Traces de cations

heavy metals: ≤0.001%

Conditions d'expédition

ambient

Température de stockage

15-25°C

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

Clé InChI

AZKSAVLVSZKNRD-UHFFFAOYSA-M

Catégories apparentées

Description générale

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Actions biochimiques/physiologiques

Cell permeable: yes
Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.
Product does not compete with ATP.
Reversible: no

Avertissement

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Autres remarques

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335,H341

Classification des risques

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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