CELLPRO-RO

Réactif de prolifération cellulaire WST-1

suitable for protein quantification, suitable for cell analysis, detection, solution

• Synonyme(s) : Réactif de prolifération cellulaire, wst-1
• NACRES: NA.21
• UNSPSC Code: 12352200

Propriétés

• form: solution
• usage: sufficient for ≤2,500 tests (11644807001), sufficient for ≤800 tests (05015944001)
• packaging: bottle of 25 mL (11644807001), bottle of 8 mL (05015944001)
• manufacturer/tradename: Roche
• storage condition: protect from light
• technique(s): protein quantification: suitable
• optimum pH: 8.0(for physiological conditions our product is buffered pH 7.3)
• λmax: 440-480 nm
• application(s): cell analysis; detection
• detection method: colorimetric
• storage temp.: −20°C

Description

General description

Sommaire
Ready-to-use solution, containing WST-1 and an electron coupling reagent.

Colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability, and cytotoxicity.

La prolifération cellulaire nécessite la réplication de l'ADN génomique. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. ′ The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.

Application

The Cell Proliferation Reagent WST-1 is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.

• Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
• Assessment of growth inhibitory antibodies and physiological mediators

Biochem/physiol Actions

The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.

Features and Benefits

• Pratique : Benefit from a ready-to-use reagent.
• Eliminate radioactive isotopes, washing steps, and additional reagents.
• Précis : The absorbance obtained strongly correlates to the cell number.
• Sensible Detect low cell numbers.
• Rapides : Process a large number of samples using a multi-well ELISA reader.

Preparation Note

Concentration de travail : μμ
μ
μμ

When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations.
However please note that the solution may become viscous.

Other Notes

Pour la recherche en sciences de la vie uniquement. Ne pas utiliser dans des procédures de diagnostic.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: No data available
• flash_point_c: No data available