Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

375095

Millipore

Heparin, Sodium Salt, Porcine Intestinal Mucosa

Synonyme(s) :

Heparin, Sodium Salt, Porcine Intestinal Mucosa, HEP, Na

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
9041-08-1
Numéro MDL:
MFCD00081689
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.79
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Description

Merck USA index - 14, 4653

Niveau de qualité

100

Forme

lyophilized

Activité spécifique

≥160 USP units/mg dry wt

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Impuretés

≤0.5 EU/mg Endotoxin

Solubilité

water: 50 mg/mL

Conditions d'expédition

ambient

Température de stockage

15-25°C

Chaîne SMILES 

[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O

InChI

1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-

Clé InChI

JRTRSJGZMRQDHI-UHFFFAOYSA-N

Description générale

M.W. 13,500-15,000. Note: 1 KU = 1000 units.
MW: 15,000 ± 2,000
Preservative-free, non-sterile. Highly sulfated dextrorotatory mucopolysaccharide with specific anticoagulant properties. Inhibits IP3-activated Ca2+ channel release of the endoplasmic reticulum. Reported to activate the ryanodine receptor. Note: 1 KU = 1000 units. MW: 15,000 ± 2,000.

Avertissement

Toxicity: Harmful (C)

Autres remarques

Seuwen, K., and Boddeke, H.G. 1995. Br. J. Pharmacol.114, 1613.
Ehrlich, B.E., et al. 1994. Trends Pharmacol. Sci.15,145.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Enrique Gómez et al.
Journal of proteome research, 19(3), 1169-1182 (2020-01-25)
Metabolomics may identify biomarkers in blood that differentiate pregnant from open embryo recipients. Fresh and vitrified/warmed, in vitro-produced embryos were transferred to Holstein recipients (discovery group). Recipient blood plasma collected on Day-0 (estrus) and Day-7 (before embryo transfer) were analyzed
Junxin Wang et al.
Bioconjugate chemistry, 29(11), 3768-3775 (2018-10-04)
We recently reported a real-time method to measure heparin in human whole blood based on the photoacoustic change of methylene blue (MB). Intriguingly, the MB behaved unlike other "turn on" photoacoustic probes-the absorbance decreased as the photoacoustic signal increased. The

Questions

1–2 of 2 Questions  

  1. I plan to use heparin in Neurobasal medium for culturing GBM cells. Literature suggests 2 µg/mL, but I need the units/mL for the final concentration. Could you provide guidance on the correct units/mL for heparin?

    1 answer

    1. Product 375095 is assayed for activity on a batch to batch basis with a specification for this product ≥160 USP units/mg dry weight. The reported activity value for this product will be found on the certificate of analysis for a given lot. It is unclear as to what activity corresponds to the provided concentration that was indicated and only the authors of the literature citation would know. Certain research articles do provide information on the activity of heparin to supplement neurobasal medium (NBM). Please see the article below which states NBM can be supplemented with 100 U/uL heparin. This decision will be at the discretion of the end user.

      https://www.sciencedirect.com/science/article/pii/S2666166723004884

      Helpful?

  2. What should be the storage temperature after 100 000 units of heparin salt is dissolved in 100 mL of PBS 1x

    1 answer

    1. Sterile filtered heparin solutions remain stable for up to 2 years when stored at 2-8 °C, provided th. Autoclaving and freezing heparin solutions are not recommended. Please see the link below to review additional information available in the datasheet for similar product H3393:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdf

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique