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05-623-Z

Sigma-Aldrich

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free

clone CTD4H8, from mouse

Synonyme(s) :

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

CTD4H8, monoclonal

Espèces réactives

mouse, yeast, rat, human

Technique(s)

ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NP_000928

Numéro d'accès UniProt

P24928

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)

Description générale

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Immunogène

Linear peptide corresponding to human RNA polymerase II.

Application

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western Blotting Analysis: 1 µg/mL from a representative lot detected RNA polymerase II in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: A 1:50 and 1:250 dilution from a representative lot detected RNA polymerase II in HeLa and A431 cells, respectively.

Qualité

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNA polymerase II in 10 µg of NIH/3T3 cell lysate.

Description de la cible

~220 kDa observed

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. (This product is Ascites Free.)

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Human cytomegalovirus (HCMV) is a ubiquitous pathogen belongs to betaherpesvirus subfamily. RNA2.7 is a highly conserved long non-coding RNA accounting for more than 20% of total viral transcripts. In our study, functions of HCMV RNA2.7 were investigated by comparison of
Liang Chen et al.
Molecular cell, 68(4), 745-757 (2017-11-07)
R-loop, a three-stranded RNA/DNA structure, has been linked to induced genome instability and regulated gene expression. To enable precision analysis of R-loops in vivo, we develop an RNase-H-based approach; this reveals predominant R-loop formation near gene promoters with strong G/C skew
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