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916293

Sigma-Aldrich

PhotoCol™-LAP

methacrylated collagen bioink kit, with LAP

Synonyme(s) :

3D Bioprinting, Bioink, Collagen

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About This Item

Code UNSPSC :
12352201
Nomenclature NACRES :
NA.23

Description

Methacrylated collagen:
Degree of methacrylation ≥ 20%
Product components :

  • Methacrylated collagen (100 mg)
  • 20 mM acetic acid (50 mL)
  • Neutralization solution (10 mL)
  • LAP photoinitiator (100 mg)

Niveau de qualité

100

Stérilité

sterile; sterile-filtered

Forme

liquid

Impuretés

≤10 EU/mL Endotoxin

Température de stockage

2-8°C

Application

PhotoCol™-LAP bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS, which allows blue light photocrosslinking of the printed structure at 405 nm. PhotoCol™-LAP provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.

Informations légales

PhotoCol is a trademark of Advanced BioMatrix, Inc.

Code de la classe de stockage

10 - Combustible liquids

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A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues.
Stoecklin, Celine, et al.
Advanced Biosystems, 1700237-1700237 (2018)
Kathryn E Drzewiecki et al.
Langmuir : the ACS journal of surfaces and colloids, 30(37), 11204-11211 (2014-09-11)
Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In
Abigail Isaacson et al.
Experimental eye research, 173, 188-193 (2018-05-18)
Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However
Andrea Mazzocchi et al.
ACS biomaterials science & engineering, 5(4), 1937-1943 (2019-11-15)
Lung cancer is the leading cause of cancer-related death worldwide yet in vitro disease models have been limited to traditional 2D culture utilizing cancer cell lines. In contrast, recently developed 3D models (organoids) have been adopted by researchers to improve
Ian D Gaudet et al.
Biointerphases, 7(1-4), 25-25 (2012-05-17)
Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material
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