817501

TSKgel^® Octadecyl-2PW (5 µm) HPLC Columns

L x I.D. 15 cm × 6 mm, C18 (octadecyl) phase

• Sinónimos: TSKgel^® Reversed Phase HPLC Column (Polymer-based)
• Código UNSPSC: 41115711
• eCl@ss: 32110501

Propiedades

• Nombre del producto: TSKgel^® Octadecyl-2PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 6 mm, 5 μm particle size
• Materiales: stainless steel hardware
• Línea del producto: TSKgel^®
• fabricante / nombre comercial: Tosoh 17501
• Extensión del etiquetado: 1 meq/mL ligand density (estimate)
• Parámetros: 0-100% organic solvent; 1.5 mL/min flow rate; 120 bar max. pressure; 4-50 °C temperature
• técnicas: HPLC: suitable
• L × D.I.: 15 cm × 6 mm
• Matriz: polymer particle platform
• grupo activo de la matriz: C18 (octadecyl) phase
• tamaño de partícula: 5 μm
• tamaño de poro: 125 Å
• pH operativo: 2-12
• técnica de separación: reversed phase

Descripción

Descripción general

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Forma física

Shipped in 55% acetonitrile - 45% water.

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Información legal

TSKgel is a registered trademark of Tosoh Corporation