52977-U
HybridSPE® DPX Tip
Hamilton, Tip volume 300 μL, box of 96 tips, bed weight 30 mg
Sinónimos:
HybridSPE® DPX SPE Tips
About This Item
Productos recomendados
product name
HybridSPE® DPX Tip, weight 30 mg (bed), Hamilton, tip volume 300 μL
product line
HybridSPE®
composition
HybridSPE® sorbent
packaging
box of 96 tips
technique(s)
solid phase extraction (SPE): suitable
tip volume
300 μL
weight
30 mg (bed)
matrix active group
Zirconia-based phase
application(s)
food and beverages
compatibility
Hamilton
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General description
In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.
The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.
We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.
What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 μL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 μL.
Guideline for 30 mg tips - plasma/serum sample: 30-100 μL; precipitating agent: 90-300 μL
Guideline for 50 mg tips - plasma/serum sample: 100-300 μL; precipitating agent: 300-900 μL
Features and Benefits
- Minimal elution solvent volumes
- Rapid extraction times (less than 3 minutes/sample)
- High extraction efficiencies
- Easy to perform extractions
- Lower costs
- Higher throughput
- Minimal training required
- Environmentally friendly
- Eliminates ion suppression effects often associated with phospholipids
- Tips offered to fit many formats: Tecan, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.
Legal Information
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenido relacionado
Solid phase extraction purifies samples for chromatographic analysis, improving data quality and protecting analytical columns.
La extracción en fase sólida (SPE) es una técnica utilizada para la preparación y la purificación rápidas y selectivas de la muestra antes del análisis cromatográfico (p. ej. HPLC, GC, TLC). La SPE se utiliza para cambiar matrices de muestra, concentrar analitos y eliminar interferencias y contaminantes con el fin de mejorar los datos y proteger la columna analítica.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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