Products with a high water content away from "the generally known reaction partners of water." This caution is due to water's potential to react violently with numerous substances, resulting in the development of gases and the ignition of released Hydrogen, posing a risk of explosion.
Additionally, various substances or mixtures can explode upon the addition of a drop of water, with details of these reactions provided in the safety data sheets of the specific reaction partners. Substance groups that react violently with water include Alkaline earth metals, Alkaline metals, Aluminum alkalines, Metallic or Nonmetallic hydrides, Metallic or Nonmetallic oxides, Acid anhydrides, and Acid chlorides.
V5265
Crystal Violet Solution
1%, aqueous solution
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
250 mL | Fecha estimada de envío25 de mayo de 2026desdePUDAHUEL | $63.400 |
500 mL | Comprobar disponibilidad del carrito | $84.800 |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C25H30ClN3
Número CAS:
Peso molecular:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3580948
Servicio técnico
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aqueous solution
Quality Segment
concentration
1%
technique(s)
microbe id | staining: suitable
color
deep violet, purple
Amax
0.36-0.44 at 589-594 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
InChI key
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
General description
Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.
Application
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.[1]
- Crystal violet is commonly used in Gram staining for the classification of bacteria.
- It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
- It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.
- Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.
- It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.[2][3]
Biochem/physiol Actions
Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.
The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.
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Este artículo | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| color deep violet, purple | color green to very dark green | color green to very dark green | color - |
| concentration 1% | concentration - | concentration - | concentration - |
| form aqueous solution | form powder | form powder | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) cleaning products |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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What does "generally known reaction partners of water" refer to in section 10.3 of the V5265 SDS? Additionally, in the SDS for a similar product, C6158, states that violent reactions are possible with strong oxidizing agents, strong acids, and oxidizing agents.
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What solvent is the crystal violet diluted in? Can it be used for staining cells for colony formation assay directly or will fixation with methanol/ethanol be needed prior to the staining?
1 answer-
This product is a 1% solution of Crystal Violet in water. For colony formation assays, it is more common to dissolve the crystal violet in a methanol-based solution. However, it is possible to fix the cells in methanol for 10-15 minutes prior to staining cells with an aqueous Crystal Violet Solution. Some sources recommend substituting absolute ethanol for methanol. Following fixation, the cells can be stained with aqueous Crystal Violet solution.
Helpful?
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What is the shelf life / expiry of this solution?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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