T9784

Tricine

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Sinónimos: N-[Tris(hydroxymethyl)methyl]glycine
• Fórmula lineal: (HOCH₂)₃CNHCH₂CO₂H
• Número de CAS: 5704-04-1
• Peso molecular: 179.17
• Beilstein: 1937804
• Número CE: 227-193-6
• Número MDL: MFCD00004277
• Código UNSPSC: 12161700
• ID de la sustancia en PubChem: 24900603
• NACRES: NA.25

Propiedades

• Línea del producto: BioXtra
• Nivel de calidad: 400
• Ensayo: ≥99% (titration)
• Formulario: powder
• impurezas: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• residuo de ign.: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• intervalo de pH útil: 7.4-8.8
• pKa (25 °C): 8.1
• solubilidad: H₂O: 1 M, clear, colorless
• trazas de anión: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• trazas de catión: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorción: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• aplicaciones: diagnostic assay manufacturing
• cadena SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Clave InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Descripción

Descripción general

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.[1]

Aplicación

Buffer component for separation of low molecular weight peptides.

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection[2]
• as a component of lactotroph feeding medium for culturing lactotrophs[3]
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer[4]

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)