T7254

N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

• Sinónimos: (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride
• Fórmula empírica (notación de Hill): C₁₄H₂₁ClN₂O₃S · HCl
• Número de CAS: 4272-74-6
• Peso molecular: 369.31
• Beilstein: 7106867
• Número CE: 224-266-4
• Número MDL: MFCD00065395
• Código UNSPSC: 12352202
• ID de la sustancia en PubChem: 24278752
• NACRES: NA.77

Propiedades

• Nombre del producto: N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
• origen biológico: synthetic (organic)
• Nivel de calidad: 200
• Ensayo: ≥96% (TLC)
• Formulario: powder
• potencia: 10-100 μM effective concentration
• solubilidad: H₂O: 50 mg/mL
• temp. de almacenamiento: −20°C
• cadena SMILES: Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
• Clave InChI: YFCUZWYIPBUQBD-ZOWNYOTGSA-N
• Información sobre el gen: human ... NFKB1(4790) , NFKB2(4791)

Descripción

Aplicación

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:

• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
• as a protease inhibitor in tissue and cell homogenization
• as a trypsin inhibitor to determine the specific protease activity levels

Acciones bioquímicas o fisiológicas

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Envase

Bottomless glass bottle. Contents are inside inserted fused cone.

Precaución

Stable at least two years when stored desiccated at −20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves