T6455

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

• NACRES: NA.47
• UNSPSC Code: 12352107

Propiedades

• biological source: synthetic
• Quality Segment: 100
• pH: 10
• application(s): hematology; histology
• storage temp.: 2-8°C

Descripción

Application

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Preparation Note

Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

Other Notes

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable