SRP3109
MCP-1/MCAF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinónimos:
CCL2, MCAF, Monocyte Chemotactic Protein-1
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Ensayo
≥98% (HPLC)
≥98% (SDS-PAGE)
Formulario
lyophilized
mol peso
8.6 kDa
envase
pkg of 20 μg
técnicas
cell culture | mammalian: suitable
impurezas
<0.1 EU/μg endotoxin, tested
color
white to off-white
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... CCL2(6347)
Descripción general
Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF), also called the C-C motif chemokine ligand 2 (CCL2), is an inflammatory cytokine. The CCL2 gene is mapped to human chromosome 17q12. CCL2 is present in epithelial, endothelial, mesangial and microglial cells. Structurally, it dimerizes and has four regions of the ß-sheet. Recombinant human MCP-1 is a 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues. The MCP family cross-reacts across species.
Aplicación
Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) has been used:
- as a chemokine supplement in the alpha minimal essential medium for culturing neurons to test its effect on JmjC histone demethylase (Jmjd2a) mRNA
- as a component of Dulbecco′s Modified Eagle (DMEM) medium, as a positive chemoattractant control in HeLa cells for migration assay
- as a chemoattractant to induced macrophage chemotaxis
Acciones bioquímicas o fisiológicas
Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) acts as a human immunodeficiency virus 1 (HIV-1) suppressor. The MCP-1 acts as a chemoattractant for T cells and natural killer (NK) cells. It also plays a key role in the migration and infiltration of macrophages. CCL2 shows under expression in high-grade ovarian serous carcinomas (HGOSCs) and is expressed less in epithelial ovarian cancer (EOC).
Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) is a member of CC chemokine family. It triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. It is found to be overexpressed in cases of atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis, and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.
Secuencia
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
Forma física
Lyophilized with no additives.
Reconstitución
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Expression of monocyte chemoattractant protein-1 in Kawasaki disease: the anti-inflammatory effect of gamma globulin therapy.
Asano T and Ogawa S
Scandinavian Journal of Immunology, 51, 98-103 (2000)
P Wojnarowicz et al.
Oncogenesis, 1, e27-e27 (2012-01-01)
The frequent loss of heterozygosity of chromosome (Chr) 17 in epithelial ovarian cancer (EOC), particularly high-grade ovarian serous carcinomas (HGOSCs), has been attributed to the disruption of known tumour suppressor genes, such as TP53 (17p13), as well as other genes
Satish L Deshmane et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 29(6), 313-326 (2009-05-16)
Chemokines constitute a family of chemoattractant cytokines and are subdivided into four families on the basis of the number and spacing of the conserved cysteine residues in the N-terminus of the protein. Chemokines play a major role in selectively recruiting
Yang Xiang et al.
Clinical epigenetics, 14(1), 32-32 (2022-03-02)
Recent studies have suggested that folic acid can restore abnormal DNA methylation and monocyte subset shifts caused by hyperhomocysteinemia (HHcy) and hyperlipidemia (HL). However, the exact mechanism of action is still not fully understood. In this study, we further investigated
A W S de Souza et al.
Clinical and experimental immunology, 178(2), 270-278 (2014-07-24)
The objective of this study is to evaluate urinary high mobility group box 1 (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with urinary CD4(+) effector memory T cells and
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