SAE0098
Lyticase from Arthrobacter luteus
free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Sinónimos:
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
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Specific activity:
≥2000 units/mg protein
Biological source:
bacterial (Arthrobacter luteus)
Servicio técnico
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Permítanos ayudarlebiological source
bacterial (Arthrobacter luteus)
Quality Segment
form
lyophilized powder
specific activity
≥2000 units/mg protein
feature
DNA free
technique(s)
cell based assay: suitable
input
plant, fungi and insect sample(s)
plant, fungi and insect null
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
General description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Other Notes
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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