S7571

Superóxido dismutasa from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

• Sinónimos: SOD, Superóxido: superóxido oxidorreductasa
• Número CAS: 9054-89-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-943-0
• MDL number: MFCD00132404
• Número CE: 1.15.1.1 (BRENDA, IUBMB)
• Specific activity: ≥3,000 units/mg protein
• Biological source: bovine

Propiedades

• biological source: bovine
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥3,000 units/mg protein
• mol wt: 32.5 kDa
• composition: Protein, ≥95% biuret
• storage condition: (Store under nitrogen.; Tightly closed. Dry.)
• technique(s): immunoblotting: suitable, inhibition assay: suitable
• color: blue-green
• pI: 4.95
• solubility: water: 20 mg/mL, aqueous buffer, pH 7.5: soluble
• UniProt accession no.: P00442, P41976
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: cow ... SOD1(281495), SOD2(281496)

Descripción

General description

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms.[1] Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.[2]

Application

Superoxide dismutase from bovine erythrocytes has been used:

• in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions[3]
• in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction[4]
• to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes[5]
• in combination with catalase to study its effect on cell differentiation in vitro
• to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Biochem/physiol Actions

La superóxido dismutasa de los eritrocitos bovinos cataliza la dismutación de los radicales superóxido a peróxido de hidrógeno y oxígeno molecular. Actúa como antioxidante y desempeña un papel fundamental en la defensa de las células contra los efectos tóxicos de los radicales de oxígeno. Compite con el óxido nítrico (NO) por el anión superóxido (que reacciona con el NO para formar peroxinitrito), por tanto la SOD promueve la actividad del NO. También se ha demostrado que la SOD suprime la apoptosis en folículos ováricos de rata cultivados, líneas celulares neuronales y ratones transgénicos.

Physical form

Lyophilized powder, essentially salt-free

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)