P9172

Pyronin Y

for NA electrophoresis

• Sinónimos: Pyronin Y, Pyronin G, Pyronin J
• Fórmula empírica (notación de Hill): C₁₇H₁₉ClN₂O
• Número de CAS: 92-32-0
• Peso molecular: 302.80
• Número de índice de color: 45005
• Beilstein: 3795789
• Número CE: 202-147-8
• Número MDL: MFCD00011725
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 57654569
• NACRES: NA.52

Propiedades

• grado: for molecular biology
• Formulario: powder
• composición: Dye content, ≥45%
• técnicas: nucleic acid detection: suitable
• mp: 250-260 °C (lit.)
• solubilidad: water: 10 mg/mL, dark red
• idoneidad: suitable for nucleic acid staining
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• Clave InChI: INCIMLINXXICKS-UHFFFAOYSA-M

Descripción

Aplicación

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
• staining of CD34⁺ cells

Acciones bioquímicas o fisiológicas

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Nota de preparación

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)