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P4929

Poly(deoxyinosinic-deoxycytidylic) acid sodium salt

double-stranded alternating copolymer

Sinónimos:

Poly(dI-dC) • Poly(dI-dC) sodium salt

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A ustedes/SKUDisponibilidadPrecio
5 units
Comprobar disponibilidad del carrito
$294.000
10 units
Comprobar disponibilidad del carrito
$465.000
25 units
Comprobar disponibilidad del carrito
$929.000

Acerca de este artículo

Número CAS:
118578-37-3
UNSPSC Code:
41106305
NACRES:
NA.51
MDL number:
MFCD00131970
Biological source:
synthetic (organic)
Form:
powder
Storage temp.:
−20°C

$294.000

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biological source

synthetic (organic)

Quality Segment

200

form

powder

storage temp.

−20°C

Application

Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in the electrophoretic mobility shift assay in p65 protein and neuro-2A protein lysate. It has also been used as a substrate in the protein arginine methyltransferase 1 (PRMT1) and DNA methyltransferase 1 (DNMT1) selectivity assay.
Poly(deoxyinosinic-deoxycytidylic) acid (Poly(dI-dC) • Poly(dI-dC)) is an alternating copolymer used as a DNA substrate for evaluation of DNA methytransfeases, such as DNA-methyltransferase 1 and as double-stranded DNA model for conformational studies of DNA structure dynamics and drug, small molecule, interactions.
Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in a study that synthesized and characterized bis(2-(pyrimidin-2-γl)ethoxy)alkanes and their pharmacological activity. Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has also been used in a study that investigated the growth of calcium carbonate films on LB/LbL matrices.

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used in electrophoretic mobility shift assay (EMSA).

Other Notes

Double-stranded alternating copolymer
One unit will yield an A260 of 1.0 in 1.0 ml of 20 mM sodium phosphate/100 mM NaCl, pH 7.0 (1 cm light path)

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. How to prepare the product of P4929-5U to 1ug/ul? What's the weight (mg), and molecular weight (g/mol) of this product?

    1 answer

    1. The polymer size of this material varies from lot to lot. The typical low range is 150 to 550 base pairs and the typical high range is 3000 to 5000 base pairs. The bulk of the product ranges from 1200 to 3000 base pairs. The molecular weight of a single Poly(dI-dC) base pair is 639.41 g/mol, resulting in an approximate molecular weight range of 767.2 kDa – 1,918.2 kDa for most of the compound. The unit per milligram value may range from 8 – 14 units per milligram and the vial content may range from 5.0 – 7.5 units. The specific vial content is lot specific and may range from 535 – 625 ug per vial. To prepare a 1 ug/ul solution, please see the link below to review the lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/p4929#product-documentation

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  2. Should I heat the product P4929-25U after resuspension to make aliquots? If yes, then how long and at what temperature? Thank you!

    1 answer

    1. This product is soluble in water or neutral buffer. Solubility is no longer considered part of the specifications, but it was previously reported to be soluble at 20 mg/mL in 0.1 M Tris-HCl, pH 8.8. Heat is not necessary to solubilize the product; dissolving in an appropriate solvent is the recommended method for resuspension.

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  3. Is Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC), stable in solution?

    1 answer

    1. Both the solution and solid are very stable and can be stored for extended periods (years) at -20 °C.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. How many milligrams of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt, do I have?

    1 answer

    1. The specifications for the product is approximately 13 U/mg, but the exact amount is lot specific.  One unit will yield an A260 of 1.0 in 1.0ml of water.  For example, the 5 unit size has approximately 0.384 mg.

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  6. What is the solubility of Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC)?

    1 answer

    1. This product is soluble in water or neutral buffer at 20 mg/ml.

      Helpful?

  7. What is the molecular weight of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt?

    1 answer

    1. Molecular weight: Polymer size (by agarose gel electrophoresis) is lot dependent with a lower range of less than 150 to 550 base pairs and a high range of 3000 to over 5000 base pairs. Most of the material is between 1200 to 3000 base pairs.

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