The Cartagena Protocol is not applicable to this product. While the manufacuturing process is proprietary, current methods of extraction and purification would eliminate the presence of any whole, vialble organisms. This is not a sterile product. Prepared solutions may be filter-sterilized prior to use.
P4912
Phospholipase D from Streptomyces sp.
Type VII, lyophilized powder, ≥150 units/mg solid
Sinónimos:
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
250 units | Comprobar disponibilidad del carrito | $283.000 |
500 units | Comprobar disponibilidad del carrito | $422.000 |
1000 units | Comprobar disponibilidad del carrito | $731.000 |
Acerca de este artículo
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
Número CE:
Specific activity:
≥150 units/mg solid
Biological source:
Streptomyces sp.
$283.000
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarlebiological source
Streptomyces sp.
Quality Segment
type
Type VII
form
lyophilized powder
specific activity
≥150 units/mg solid
storage temp.
−20°C
Application
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.
Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
Biochem/physiol Actions
Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Analysis Note
Protein by biuret
Other Notes
One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.
1 of 1
Este artículo | |||
|---|---|---|---|
| description Type VII, lyophilized powder, ≥150 units/mg solid | description ≥50,000 units/mL, buffered aqueous glycerol solution | description Type IV, lyophilized powder, ≥100 units/mg solid | description Type II, lyophilized powder, ≥60 units/mg protein |
| specific activity ≥150 units/mg solid | specific activity ≥50,000 units/mL | specific activity ≥100 units/mg solid | specific activity ≥60 units/mg protein |
| biological source Streptomyces sp. | biological source - | biological source - | biological source Arachis hypogaea |
| form lyophilized powder | form buffered aqueous glycerol solution | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| type Type VII | type - | type Type IV | type Type II |
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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