Drying should proceed at room temperature for at least 2 hours before introducing cells and culture medium. No additional drying conditions are specified. For coating, the recommended volume is 1.0 mL per 25 cm2 of culture surface area, which is 0.04 mL/cm2 (40 µL/cm2). Distribute the solution evenly across the surface by gentle rocking to ensure uniform coating. End users may prefer to optimize drying time and temperature internally to meet experimental or application-specific requirements. A general coating protocol is available for review at the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/poly-lysine-product
P4707
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Sinónimos:
Disolución de PLL
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
50 mL | Comprobar disponibilidad del carrito | $131.000 |
Acerca de este artículo
Biological source:
synthetic
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.01%
Mol wt:
70—150 kDa
$131.000
Comprobar disponibilidad del carrito
Utilice el código promocional TXA en el carrito para aplicar un descuento sobre este producto.
Servicio técnico
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Permítanos ayudarleNombre del producto
Poli-L-lisina solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
biological source
synthetic
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
liquid
mol wt
70—150 kDa
packaging
pkg of 50 mL
concentration
0.01%
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
impurities
endotoxin, tested
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
General description
La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.
Application
Los polímeros de poli-L-lisina pueden utilizarse en la promoción de la adhesión celular a los sustratos sólidos, la conjugación al metotrexato para incrementar el transporte de medicamentos, la microencapsulación de islote, la tecnología de microencapsulación celular, recubrimiento de portaobjetos de vidrio de micromatrices y preparaciones cromosómicas. La poli-L-lisina de menor peso molecular (30 000-70 000) es menos viscosa en disolución, pero las versiones de mayor peso molecular proporcionan mas sitios de unión por molécula.
- Se han utilizado cubreobjetos de vidrio recubiertos con poli-L-lisina para montar muestras para el microscopio electrónico de barrido.
- Se utilizaron pocillos recubiertos con poli-L-lisina para atrapar células PC-12 en los electrodos.
- Se utilizaron portaobjetos de vidrio borosilicato recubiertos con poli-L-lisina para la siembra de células transducidas con vectores víricos.
Biochem/physiol Actions
La poli-L-lisina es un factor de unión inespecífica a las células útil en la promoción de la adhesión celular a los sustratos sólidos intensificando la interacción electrostática entre iones con carga negativa de la membrana celular y la superficie del cultivo. Cuando se absorbe a la superficie del cultivo celular, la poli-L-lisina funciona aumentando el número de sitios con carga positiva disponibles para la unión a la célula. Con las células que pueden digerir la poli-L-lisina, debe utilizarse la poli-D-lisina como factor de fijación.
Preparation Note
Este producto se suministra como una disolución al 0,01% en agua esterilizada por filtración. El recubrimiento de los portaobjetos con esta disolución debe ir seguido de una incubación de 5 minutos y de secar los portaobjetos a temperatura ambiente. Los portaobjetos recubiertos serán estables durante un año si se protegen del polvo.
Other Notes
La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.
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Este artículo | |||
|---|---|---|---|
| description 0.01%, sterile-filtered, BioReagent, suitable for cell culture | description mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture | description 0.1 % (w/v) in H2O | description mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility γ-irradiated | sterility - | sterility γ-irradiated |
| biological source synthetic | biological source synthetic (organic) | biological source synthetic | biological source synthetic (organic) |
| concentration 0.01% | concentration - | concentration 0.1 % (w/v) in H2O | concentration - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form lyophilized powder | form - | form lyophilized powder |
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Clase de almacenamiento
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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FAQによると、コーティングの際、ポリリジンを5分間適用した後に「室温で数時間」乾燥させると他の方に説明されていました。しかし、私たちは再現性のある（一貫した）結果を得たいと考えております。そのため、具体的に何時間乾燥させるべきか、またどのような条件下で乾燥させるべきか、より詳しく説明していただけないでしょうか。さらに、1 cm2あたりの適切な液量についても教えていただけますと幸いです。
1 answer-
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When coating coverslips with item P4707, is it necessary to wash the reagent off after 5 minutes, or is there a different recommended procedure?
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The product is provided as a 0.01% sterile filtered solution in water. When coating slides with this solution, it is recommended to incubate for 5 minutes and allow the slides to dry at room temperature.
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Is the product P4707 suitable for coating glassware for cell culture? Also, what is the difference between Poly L and Poly D lysine?
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Yes, the product P4707 is suitable for coating glassware for cell culture. The difference between Poly L and Poly D lysine lies in their stereoisomeric properties. Poly-L-Lysine is a charge enhancer and can be used for coating many surfaces, including tissue culture plasticware and glassware. However, certain cells can digest poly-L-lysine, in which case the poly-D-lysine, such as P7405, should be used. This product can also be used to coat tissue culture plasticware and glassware.
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How many times can it be reused?
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The number of times that this product may be re-applied has not been determined. This would be dependent on several factors, such as surface area, material type, surface pretreatment, and incubation period. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdfHelpful?
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What is the molecular weight of product P4707?
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Poly-L-lysine solution is 70-150 kDa molecular weight.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?
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A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.
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Should I use Poly-L-Lysine or Poly-D-Lysine?
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Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?
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For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.
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How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?
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Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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