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Merck
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Documentos clave

M0268

Sigma-Aldrich

Medio esencial mínimo Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nivel de calidad

500

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Aplicación

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Características y beneficios

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Cantidad

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitución

Supplement with 2.2 g/L sodium bicarbonate.

Otras notas

Essentially the same as the original formula, except it is provided as a powder.

suplemento

Referencia del producto
Descripción
Precios

también adquirido normalmente con este producto

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Descripción
Precios

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000379555
0000379554
0000379552
0000378895
0000288021

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T L Freeman et al.
Biochemical pharmacology, 68(2), 223-230 (2004-06-15)
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The poor mechanical properties and processability of pristine heterocyclic conductive polymers represent the most notable scientific and technological challenges that have greatly limited the application of these polymers. We report a soft and mechanically processable free-standing pristine polypyrrole (PPy) membrane
G F Shiguemoto et al.
Brazilian journal of biology = Revista brasleira de biologia, 81(2), 301-308 (2020-07-23)
Wild fish domestication can be considered a strategic approach to endangered species conservation, supporting studies and reducing economic and environmental costs. Three of the most important strategies in the domestication processes of fish are the adaptation of wild fish to
Xiaolong Sun et al.
Acta histochemica, 122(8), 151649-151649 (2020-11-10)
As one of the most frequent diabetic complications, diabetic foot ulcer (DFU) can cause limb ischemia or even amputation. Paeoniflorin (PF) has been reported to possess many kinds of biological functions, such as antioxidant and anti-inflammatory effects. However, the role
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PloS one, 11(3), e0150687-e0150687 (2016-03-02)
The metabolism of S. Typhimurium within infected host cells plays a fundamental role in virulence since it enables intracellular proliferation and dissemination and affects the innate immune response. An essential requirement for the intracellular replication of S. Typhimurium is the
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Artículos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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