L2137
Lipopolysaccharides from Salmonella enterica serotype minnesota
purified by gel-filtration chromatography
Sinónimos:
LPS
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About This Item
Productos recomendados
biological source
Salmonella enterica (Serotype minnesota)
Quality Level
form
lyophilized powder
purified by
gel-filtration chromatography
impurities
<1% Protein
color
white
solubility
water: 4.90-5.10 mg/mL, cloudy to hazy, colorless
shipped in
ambient
storage temp.
2-8°C
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General description
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
This product is extracted from Salmonella Minnesota and purified by gel filtration. The source strain is ATCC 9700. Mechanisms of complement activation by LPS of both rough and smooth strains of Salmonella Minnesota were studied by immunoassay of the activation products.
Application
- TRPV4 is not the molecular sensor for bacterial lipopolysaccharides-induced calcium signaling: This study explores the signaling pathways activated by lipopolysaccharides from Salmonella enterica serotype Minnesota, demonstrating that TRPV4 is not involved, advancing understanding of immune responses to bacterial endotoxins (Wang et al., 2023).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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The Journal of biological chemistry, 288(35), 25362-25374 (2013-07-16)
Broad-spectrum inhibitors of histone deacetylases (HDACs) constrain Toll-like receptor (TLR)-inducible production of key proinflammatory mediators. Here we investigated HDAC-dependent inflammatory responses in mouse macrophages. Of the classical Hdacs, Hdac7 was expressed at elevated levels in inflammatory macrophages (thioglycollate-elicited peritoneal macrophages)
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Phosphoinositide 3-kinase (PI3K) p110 isoforms are membrane lipid kinases classically involved in signal transduction. Lipopolysaccharide (LPS)-activated macrophages constitutively and abundantly secrete proinflammatory cytokines including tumor necrosis factor-α (TNF). Loss of function of the p110δ isoform of PI3K using inhibitors, RNA-mediated
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Artículos
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Contenido relacionado
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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