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I3390

IMDM

Name: IMDM
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217469

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Propiedades

Nombre del producto

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Descripción

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Supplement with 0.584 g/L L-glutamine

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Este artículo	I6529	I7633	FG0465
Sigma-Aldrich I3390 Iscove′s Modified Dulbecco′s Medium	SAFC I6529 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich I7633 Iscove′s Modified Dulbecco′s Medium	SAFC FG0465 Iscove′s Modified Dulbecco′s Medium
description liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma	description With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma	description With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture	description With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable, cell culture \| hybridoma: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form powder	form liquid
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile, sterile-filtered
components phenol red: yes L-glutamine: no glucose: yes	components glucose: yes phenol red: yes NaHCO₃: yes L-glutamine: yes	components phenol red: yes glucose: yes L-glutamine: yes	components stable glutamine: yes NaHCO₃: yes glucose: yes phenol red: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Clase de almacenamiento

12 - Non Combustible Liquids

¿Qué está pasando?

WGK 1

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

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