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H8162

Millipore

HIS-Select® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Sinónimos:

cobalt affinity gel, cobalt charged agarose

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

Nivel de calidad

200

Formulario

(1:1 suspension in a 30% ethanol solution)

caducidad

1 yr (when stored properly.)

técnicas

protein purification: suitable

Matriz

6% Beaded Agarose

capacidad

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

temp. de almacenamiento

2-8°C

Descripción general

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Aplicación

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select® Cobalt Affinity Gel has been used to purify His-tagged proteins.

Forma física

1:1 suspension in a 30% ethanol solution

Información legal

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Palabra de señalización

Danger

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
0000387154
0000387154
0000289331
0000360616
0000360616

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Retromer associates with the cytoplasmic amino-terminus of polycystin-2
Tilley F, et al.
Journal of Cell Science, 211342-211342 (2018)
Michael Riis Hansen et al.
Biomolecular NMR assignments, 8(1), 103-108 (2013-01-15)
The type I phosphoribosyltransferase OMP synthase (EC 2.4.2.10) is involved in de novo synthesis of pyrimidine nucleotides forming the UMP precursor orotidine 5'-monophosphate (OMP). The homodimeric enzyme has a Rossman α/β core topped by a base-enclosing "hood" domain and a
Adam S B Jalal et al.
Cell reports, 32(3), 107928-107928 (2020-07-23)
Specific interactions between proteins and DNA are essential to many biological processes. Yet, it remains unclear how the diversification in DNA-binding specificity was brought about, and the mutational paths that led to changes in specificity are unknown. Using a pair
Katie L Whalen et al.
Journal of chemical information and modeling, 53(9), 2349-2359 (2013-10-12)
Glutamate racemase (GR) is a cofactor independent amino acid racemase that has recently garnered increasing attention as an antimicrobial drug target. There are numerous high resolution crystal structures of GR, yet these are invariably bound to either D-glutamate or very
Kurt C Curtis et al.
Parasitology research, 120(2), 535-545 (2021-01-09)
Paragonimiasis is a foodborne trematode infection that affects 23 million people, mainly in Asia. Lung fluke infections lead frequently to chronic cough with fever and hemoptysis, and are often confused with lung cancer or tuberculosis. Paragonimiasis can be efficiently treated
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