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G9145

[Leu15]-Gastrin I human

≥95% (HPLC)

Sinónimos:

[Leu15]-HG-17, LHG-17, [15-Leucine]-Gastrin I, human heptadecapeptide, [18-Pyroglutamic acid, 32-leucine]-Big gastrin I-(18-34)-peptide amide, human, [Leu15]-Gastrin I, human, [Leucine15]-Gastrin I heptadecapeptide, [Leucine15]-Little gastrin I

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A ustedes/SKUDisponibilidadPrecio
0.1 mg
Comprobar disponibilidad del carrito
$178.000
0.5 mg
Comprobar disponibilidad del carrito
$302.000

Acerca de este artículo

Fórmula empírica (notación de Hill):
C98H126N20O31
Número CAS:
39024-57-2
Peso molecular:
2080.16
UNSPSC Code:
12352209
NACRES:
NA.32
PubChem Substance ID:
24895364
EC Number:
254-250-2
Beilstein/REAXYS Number:
5227423
MDL number:
MFCD00076502
Form:
powder
Assay:
≥95% (HPLC)
Biological source:
human

$178.000

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biological source

human

Quality Segment

200

assay

≥95% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P01350

storage temp.

−20°C

SMILES string

CC(C)CC(NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C3CCCN3C(=O)CNC(=O)C4CCC(=O)N4)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(C)C(=O)NC(Cc5ccc(O)cc5)C(=O)NCC(=O)NC(Cc6c[nH]c7ccccc67)C(=O)NC(CC(C)C)C(=O)NC(CC(O)=O)C(=O)NC(Cc8ccccc8)C(N)=O

InChI

1S/C98H126N20O31/c1-49(2)38-68(115-96(147)72(43-55-46-101-60-19-12-10-17-58(55)60)117-98(149)74-20-13-37-118(74)77(122)48-103-86(137)61-25-31-75(120)105-61)93(144)111-66(30-36-82(131)132)92(143)110-65(29-35-81(129)130)91(142)109-64(28-34-80(127)128)90(141)108-63(27-33-79(125)126)89(140)107-62(26-32-78(123)124)88(139)104-51(5)85(136)113-70(41-53-21-23-56(119)24-22-53)87(138)102-47-76(121)106-71(42-54-45-100-59-18-11-9-16-57(54)59)95(146)114-69(39-50(3)4)94(145)116-73(44-83(133)134)97(148)112-67(84(99)135)40-52-14-7-6-8-15-52/h6-12,14-19,21-24,45-46,49-51,61-74,100-101,119H,13,20,25-44,47-48H2,1-5H3,(H2,99,135)(H,102,138)(H,103,137)(H,104,139)(H,105,120)(H,106,121)(H,107,140)(H,108,141)(H,109,142)(H,110,143)(H,111,144)(H,112,148)(H,113,136)(H,114,146)(H,115,147)(H,116,145)(H,117,149)(H,123,124)(H,125,126)(H,127,128)(H,129,130)(H,131,132)(H,133,134)

InChI key

CMVMLPDUAGUTOC-UHFFFAOYSA-N

Gene Information

human ... GAST(2520)

General description

GAST gene encodes for the protein [Leu15]-Gastrin I in human. In human chromosome, the gene GAST is localized on 17q21.2. G cells in the stomach antrum produces the precursor of [Leu15]-Gastrin I, progastrin. Progastrin undergoes cleavage and processing to yield gastrin, which is trophic for the entire gastrointestinal epithelium. Gastrin is essential for the growth of the digestive system and stimulates the production of gastric acid by parietal cells. Gastrin exerts its function through G-protein-coupled receptor called the cholecystokinin (CCK) or CCK-B receptor (CCK-BR). Gastrin release is stimulated by food, especially protein diet and is inhibited by very low pH. Atrophic gastritis, a Helicobacter pylori infection and long-term administration of proton pump inhibitors infection may cause overexpression of gastrin. Gastric adenocarcinoma show high levels of gastrin.

Application

Gastrin has been used:
  • as a medium supplement establish enteroid cultures
  • as a supplement to standard Dulbecco′s Modified Eagle Medium (DMEM) culture medium, human minigut medium to establish the growth of crypts.

Biochem/physiol Actions

Gastrin I analog with full biological activity and improved stability in aqueous solutions. Leu15 replaces Met15, that is readily oxidized resulting in loss of biological activity.

Other Notes

Lyophilized from 0.1% TFA in H2O

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Este artículo
G9020I3769G1276
[Leu15]-Gastrin I human ≥95% (HPLC)

Sigma-Aldrich

G9145

[Leu15]-Gastrin I human

Gastrin I human ≥95% (HPLC), powder

Sigma-Aldrich

G9020

Gastrin I human

Insulin-like Growth Factor-I human IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

I3769

Insulin-like Growth Factor-I human

Gastrin I rat ≥97% (HPLC)

Sigma-Aldrich

G1276

Gastrin I rat

biological source

human

biological source

human

biological source

human

biological source

rat

technique(s)

cell culture | mammalian: suitable

technique(s)

protein expression: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥97% (HPLC)

form

powder

form

powder

form

lyophilized powder

form

-

UniProt accession no.

P01350

UniProt accession no.

P01350

UniProt accession no.

P05019

UniProt accession no.

P04563

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. Could you please provide information regarding the storage duration?

    1 answer

    1. This product is assigned a retest date of 5 years from the date of quality release. A firm expiration date is not assigned. The retest date is lot specific and available in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/g9145#product-documentation

      The solution stability of this material has not been investigated. In general, peptide solutions may be stable up to 1 week when stored at 2-8°C. Should long term storage be necessary, storing in aliquots at -20°C is suggested. Avoid repeated freeze/thaw cycles. Note that peptides have a tendency to stick to plastic containers upon storage.

      Helpful?

  2. Can you reconstitute the powder directly into 0.1 % BSA/PBS?

    1 answer

    1. This product is tested for solubility at 1 mg/ml in 0.1% NaOH or 1% NH4OH. Other solvents have not been tested in-house. This product was lyophilized from 0.1% TFA, which is a strong acid. Reconstituting in 0.1% NaOH would help to neutralize the acid. Reconstituting in PBS will not damage the gastrin, but the solution may still be acidic. There may be instances where the gastrin is more difficult to resuspend in PBS.

      Helpful?

  3. Is the powder sterile for cell culture use

    1 answer

    1. This item is not a sterile product. Stock solutions may be filter sterilized.

      Helpful?

  4. After preparing aliquots, what is the stability of this product at -20?

    1 answer

    1. The stability of this product in solution has not been evaluated. Generally, peptides should not be stored in solution for extended periods. Should solution storage be necessary, storing aliquots at -20°C is suggested. Note that peptides have a tendency to stick to plastic containers upon storage.

      Helpful?

  5. Is this product also suitable for use with other mammalian species, such as mouse, for ex vivo tissue studies, as opposed to just human organoid culture medium?

    1 answer

    1. The product's validation for batch release does not currently cover murine ex vivo tissue testing. However, the following citations may provide insights into its application for ex vivo non-human samples:

      Sci Adv. 2023 Nov 17;9(46):eadh0322. Title: Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells PMID: 37967175

      Infect Immun. 2016 Sep 19;84(10):3083-91. Title: Intestinal Enteroids Model Guanylate Cyclase C-Dependent Secretion Induced by Heat-Stable Enterotoxins PMID: 27481254

      Helpful?

  6. I am interested in this product. A colleague uses it for human organoid culture medium. However, I work with murine ex vivo tissues. Is this also applicable for other mammalian species (so mouse in stead of human)?

    1 answer

    1. This product has not been tested for use with murine ex vivo tissue. However, the following citations may be of interest for its application in ex vivo non-human samples.

      Sci Adv. 2023 Nov 17;9(46):eadh0322.
      Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells
      PMID: 37967175
      https://pubmed.ncbi.nlm.nih.gov/37967175/

      Infect Immun. 2016 Sep 19;84(10):3083-91.
      Intestinal Enteroids Model Guanylate Cyclase C-Dependent Secretion Induced by Heat-Stable Enterotoxins
      PMID: 27481254
      https://pubmed.ncbi.nlm.nih.gov/27481254/

      Helpful?

  7. この製品の溶媒は何を用いればよろしいでしょうか。水またはPBS(-)には溶けますでしょうか、あるいはDMSOでしょうか。

    1 answer

    1. This product is tested for solubility at 1mg/ml in 0.1% NaOH or 1% NH4OH. Other solvents have not been evaluated.

      Helpful?

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