G5760

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

• Sinónimos: G-6-P-DH
• Número de CAS: 9001-40-5
• Comisión internacional de enzimas: 1.1.1.49 (BRENDA, IUBMB)
• Número CE: 232-602-6
• Número MDL: MFCD00081656
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Leuconostoc mesenteroides)
• Nivel de calidad: 200
• tpo: Type XXIII
• Formulario: ammonium sulfate suspension
• actividad específica: 550-1,100 units/mg protein (biuret)
• mol peso: 54 kDa
• concentración: ≥2.0 mg/mL Biuret
• actividad extraña: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.

Aplicación

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:

• as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity
• as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes
• in the glucose-phosphorylating activity of chloroplast extracts
• as a model to test the effect of seed protein fractions on enzyme protection during dehydration.
• in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.

Acciones bioquímicas o fisiológicas

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm. G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD⁺. G6PD uses NAD⁺ or NADP⁺ based on the catabolic or anabolic metabolic pathway.G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.

Definición de unidad

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Forma física

Suspension in 2.7 M (NH₄)₂SO₄ solution containing 42 mM Tris and 0.8 mM MgCl₂

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)