G5166

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola

• Sinónimos: N-Glycosidase F, Peptide N-glycosidase
• Número CAS: 83534-39-8
• UNSPSC Code: 12352204
• NACRES: NA.32
• Número CE: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD06656235
• Specific activity: ≥20000 units/mg protein
• Biological source: bacterial (Elizabethkingia miricola)

Propiedades

• Nombre del producto: PNGase F from Elizabethkingia miricola, buffered aqueous solution
• biological source: bacterial (Elizabethkingia miricola)
• Quality Segment: 200
• conjugate: (N-linked)
• form: buffered aqueous solution
• specific activity: ≥20000 units/mg protein
• mol wt: 36 kDa
• technique(s): protein mass spectrometry: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Application

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)