G0635

Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate

≥85% (HPLC), powder

• Sinónimos: β:γ-Imidoguanosine 5′-triphosphate trisodium salt hydrate, 5′-Guanylyl-imidodiphosphate trisodium salt hydrate, GMP-PNP, Gpp(NH)p
• Fórmula empírica (notación de Hill): C₁₀H₁₄N₆Na₃O₁₃P₃ · xH₂O
• Número de CAS: 148892-91-5
• Peso molecular: 588.14 (anhydrous basis)
• MDL number: MFCD00084770
• UNSPSC Code: 41106305
• PubChem Substance ID: 24895020
• NACRES: NA.51

Propiedades

• biological source: synthetic (organic)
• Quality Level: 200
• assay: ≥85% (HPLC)
• form: powder
• color: white
• solubility: H₂O: 50 mg/mL
• shipped in: dry ice
• storage temp.: −70°C
• SMILES string: [Na+].[Na+].[Na+].Nc1nc(O)c2ncn([C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP(O)([O-])=O)[C@@H](O)[C@H]3O)c2n1
• InChI: 1S/C10H17N6O13P3.3Na/c11-10-13-7-4(8(19)14-10)12-2-16(7)9-6(18)5(17)3(28-9)1-27-32(25,26)29-31(23,24)15-30(20,21)22;;;/h2-3,5-6,9,17-18H,1H2,(H,25,26)(H3,11,13,14,19)(H4,15,20,21,22,23,24);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
• InChI key: QGJNRMUNXAROIT-CYCLDIHTSA-K

Descripción

General description

Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.

Application

Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate has been used: to study K-RAS interactions with the membrane,[1] for the crystallization of Mycobacterium tuberculosis FtsZ,[2] as a translation initiation inhibitor for the analysis of the 48S pre-initiation complex.[3]

Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.

Biochem/physiol Actions

Guanosine 5′-[ß,γ-imido]triphosphate binds and irreversibly activates G proteins.[4] Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.[5][6]

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves