E5529
EZview™ Red Streptavidin Affinity Gel
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NACRES:
NA.56
UNSPSC Code:
41106500
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suspension
Quality Level
shelf life
1 yr
technique(s)
western blot: suitable
matrix
4% agarose
pH
7.2
capacity
~10 μg(biotin per ml of packed gel)
shipped in
wet ice
storage temp.
2-8°C
Categorías relacionadas
General description
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
Application
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenido relacionado
Investigue las interacciones proteína-proteína in vitro con análisis pull-down, utilizando métodos de afinidad, pull-down con GST, TAP y co-inmunoprecipitación.
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| E5529-5X1ML | 04061822788954 |
| E5529-1ML | 04061822788947 |
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