This product reconstituted to 0.365 g/L yields a molarity of approximately 0.0025 mol/L (2.5 mM). Please see the link below to review the full formulation:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/822/486/d0547pis-ms.pdf
D8900
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L -glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
DMEM Hams F12, DME/F-12, 1:1 mixture
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Servicio técnico
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Permítanos ayudarleQuality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:
- as a component of the culture medium for endometrial explants in vitro culture
- to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
- to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
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Este artículo | |||
|---|---|---|---|
| description With | description With | description With | description With |
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components sodium pyruvate: 0.055 g/L | components NaHCO3: no | components NaHCO3: no | components glucose: 3.15 g/L |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
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What is the molarity of L-glutamine when reconstituted?
1 answer-
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What is the molarity of glucose when reconstituted with water?
1 answer-
Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).
Helpful?
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