D1821

DAF-FM

≥98% (HPLC)

• Sinónimos: 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM
• Fórmula empírica (notación de Hill): C₂₁H₁₄F₂N₂O₅
• Número CAS: 254109-20-1
• Peso molecular: 412.34
• MDL number: MFCD29073049
• UNSPSC Code: 12352204
• PubChem Substance ID: 329798711
• NACRES: NA.77
• Assay: ≥98% (HPLC)
• Form: powder
• Quality level: 100
• Storage condition: desiccated

Propiedades

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated
• color: white to brown
• solubility: DMSO: >1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• InChI key: DIJCILWNOLHJCG-UHFFFAOYSA-N

Descripción

Application

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.[1]

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Preparation Note

Color variation within the range from white to brown does not change performance.

Disclaimer

hygroscopic

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable