R2C

89031606, rat testis, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: R2C, 89031606
• origen biológico: rat testis
• envase: tube of 5 μg 89031606-DNA-5UG; pkg of vial of cells 89031606-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat testis Leydig cell tumour

Descripción línea celular

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Medio de cultivo

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

Otras notas

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