This product is not sterile as received. Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration as we have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes can be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol. Detailed information can be found in the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
C9301
Chicken Collagen Type II
from chicken sternal cartilage, powder, suitable for cell culture
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
5 MG
$136.000
25 MG
$525.000
100 MG
$1.640.000
Seleccione un Tamaño
Cambiar Vistas
5 MG
$136.000
25 MG
$525.000
100 MG
$1.640.000
About This Item
Productos recomendados
Nombre del producto
Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
origen biológico
chicken (Sternal cartilage)
Nivel de calidad
tpo
Type II (Miller)
Línea del producto
BioReagent
Formulario
powder
envase
glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg
concentración
60-80% (biuret)
técnicas
cell culture | mammalian: suitable
cobertura de la superficie
6‑10 μg/cm2
solubilidad
acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
Nº de acceso UniProt
Especificidad de unión
Peptide Source: Fibrinogen
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Información sobre el gen
chicken ... COL2A1(395069)
Descripción general
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is made of three polypeptide chains.[1] Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1[2] chains.[1] Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye.[2] The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.[3]
Aplicación
Collagen from chicken sternal cartilage has been used:
Acciones bioquímicas o fisiológicas
Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.
Características y beneficios
This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.
Otras notas
Major component of articular cartilage.
Nota de preparación
Prepared by a modification of the method of Trentham, D.E., et al.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering
Toh W S
Methods in Molecular Biology (2014)
Blockade of the interleukin?7 receptor inhibits collagen?induced arthritis and is associated with reduction of T cell activity and proinflammatory mediators.
Hartgring S A, et al.
Arthritis and Rheumatism, 62(9), 2716-2725 (2010)
High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes.
Chen X, et al.
Cardiovascular Diabetology, 16(1), 121-121 (2017)
Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.
Hoek R M, et al.
Arthritis and Rheumatism, 62(4), 1036-1042 (2010)
Clinical Impact of Bone and Connective Tissue Markers (2012)
Artículos
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
-
Is this product sterilized? If not, how do we sterilize it? Thank you!
1 answer-
Helpful?
-
-
May I please know the exact molecular weight of the protein ?
1 answer-
The product C9301 gives 1 major band at approximately 150 KDa in the SDS-PAGE test.
Helpful?
-
-
What is the recommended method for storing stock solutions of item C9301, Collagen from chicken sternal cartilage after reconstitution?
1 answer-
Following reconstitution, store the solution in the refrigerator at 4°C. Stock solutions are stable for up to 6 months at 4°C. It is important to avoid freeze/thaw cycles of the solutions.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
