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C0858

Sigma-Aldrich

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

Sinónimos:

COX-2, Prostaglandin H synthase 2

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About This Item

MDL number:
MFCD00071378
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells

Quality Level

200

assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥8000 units/mg protein

mol wt

dimer subunit mol wt 70-74 kDa

UniProt accession no.

P35354

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTGS2(5743)

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Other Notes

Tagged with a six-residue histidine sequence near the N-terminus.

Unit Definition

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

Substrates

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Masanori Sugimoto et al.
Drug development research, 77(1), 20-28 (2016-01-15)
Preclinical Research This article describes the properties of a novel topical NSAID (Nonsteroidal anti-inflammatory drug) patch, SFPP (S(+)-flurbiprofen plaster), containing the potent cyclooxygenase (COX) inhibitor, S(+)-flurbiprofen (SFP). The present studies were conducted to confirm human COX inhibition and absorption of
Mater H Mahnashi et al.
Evidence-based complementary and alternative medicine : eCAM, 2022, 7921408-7921408 (2022-04-12)
Background. The current study aims to give a scientific origin for employing Habenaria plantaginea Lindl. as a potential candidate against nociception, inflammation, and pyrexia. The pharmacological studies were performed on crude extract and subfractions. In the gas chromatography-mass spectroscopy analysis
Alejandra Moreno et al.
Natural product communications, 10(6), 991-994 (2015-07-23)
Zuccagnia punctata Cav. (Fabaceae) is an Argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). The chalcones were identified as pigments responsible for the yellow color of the flowers. Hydroethanolic extracts were obtained both from fresh flowers and
Muhammad Saeed Jan et al.
European journal of medicinal chemistry, 186, 111863-111863 (2019-11-20)
In recent years, drug discovery paradigm has been shifted from conventional single target inhibition toward multitarget design concept. In current research, we have reported synthesis, in-vitro, in-vivo and acute toxicity determination of N-substituted pyrrolidine-2,5-dione derivatives as multitarget anti-inflammatory agents. We
María J Pérez et al.
Journal of food science, 83(5), 1454-1462 (2018-04-17)
This work is part of the search in native food matrices from arid regions of Argentina of interest to improve human health. Prosopis species are ethnic food resources in South America capable of growing in arid and semi-arid environments. This
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