AA0100

Ammonia Assay Kit

sufficient for 100 assays

• Sinónimos: Ammonia Quantitative Test Kit
• NACRES: NA.84
• UNSPSC Code: 12352202

Propiedades

• usage: sufficient for 100 assays
• Quality Segment: 200
• storage temp.: 2-8°C

Descripción

Application

This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP⁺). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.

Solo componentes del kit

• Ammonia Assay Reagent 10 vial(s)
• L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
• Ammonia Standard Solution 1 mL/vial

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H319,H334
• pcodes: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable