This product can be reconstituted in ammonium hydroxide, pH >9, to a minimum of 1 mg/mL, or in DMSO at 0.4–4 mM. Stock solutions can then be serially diluted into aqueous buffers, such as PBS or saline, to the desired working concentrations. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf
A9810
Fragmento 1-42 de la proteína amiloide β
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
0.1 mg | Comprobar disponibilidad del carrito | $239.000 |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C203H311N55O60S
Número CAS:
Peso molecular:
4514.04
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Servicio técnico
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Permítanos ayudarleassay
≥95% (HPLC)
Quality Segment
form
powder
UniProt accession no.
storage temp.
−20°C
SMILES string
S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(
InChI
1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112
InChI key
DZHSAHHDTRWUTF-SIQRNXPUSA-N
Gene Information
human ... APP(351)
General description
La proteína β amiloide se produce a partir de la proteína precursora amiloide-β (APP). Consta de dos variantes C terminales, como la Aβ 1-42 de cola larga y la Aβ 1-40 de cola corta. La APP está localizada en el cromosoma humano 21q21.3.
Application
El fragmento 1-42 de la proteína amiloide β se ha utilizado:
- para la preparación del oligómero Aβ 1-42
- para análisis mediante inmunotransferencia western
- para la prueba de interferencia del ensayo de la Aβ42 plasmática mediante reducción inmunomagnética (IMR)
- para estudiar los efectos del resveratrol sobre el deterioro inducido por la Aβ 1-42 en el aprendizaje espacial, la memoria y la plasticidad sináptica
- para investigar el efecto de la Aβ en los cultivos de células epiteliales
Biochem/physiol Actions
La fragmento predominante de la proteína β amiloide encontrado en los pacientes con enfermedad de Alzheimer y síndrome de Down.
El fragmento 1-42 de la proteína β amiloide (Aβ 1-42) tiene propiedades antioxidantes y neuroprotectoras. La acumulación de la proteína β amiloide se asocia con la enfermedad de Alzheimer (AD) y el síndrome de Down. La Aβ 1-42 regula el transporte del colesterol y puede actuar como un factor de transcripción. Puede que posea propiedades anti-inflamatorias y antimicrobianas.
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Este artículo | |||
|---|---|---|---|
| description - | description ≥95% (HPLC) | description ≥90% (HPLC), powder | description - |
| assay ≥95% (HPLC) | assay ≥95% (HPLC) | assay ≥90% (HPLC) | assay >97.0% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form powder | form powder | form powder | form (Clear dry film) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What would be the best method to reconstitute this amyloid β protein fragment 1-42 into the concentrations starting at 0.1nM and working my way up to 5, 10 and 100nM?
1 answer-
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After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?
1 answer-
This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?
1 answer-
These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
PP69 Calbiochem:
https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
A9810 Sigma-Aldrich:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution
1 answer-
This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.
Helpful?
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How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?
1 answer-
It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.
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in which solvent it should be dissolved ?
1 answer-
As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.
See the link below to review the product information sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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What is the source of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
This protein fragment is a synthetic organic. The gene for this peptide is located on human chromosome 21.
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What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992
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What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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