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Merck

A5295

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bovine

technique(s)

direct ELISA: 1:20,000
dot blot: 1:80,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

purified bovine IgG

Application

Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • bovine viral diarrhea virus (BVDV) plaque
  • focus-forming assays

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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