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A3765

Sigma-Aldrich

N-(p-Aminophenyl)oxamic acid–Agarose

saline suspension

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

form

saline suspension

Quality Level

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

20 atoms

capacity

≥37 units/mL binding capacity (neuraminidase (N2876, 4MU-NANA substrate))

storage temp.

2-8°C

Application

N-(p-Aminophenyl)oxamic acid agarose is used in affinity chromatography, protein chromatography and in specialty resins. N-(p-Aminophenyl)oxamic acid has been used in studies characterizing sialidase, neuraminidase, β-N-acetylhexosaminidases and β-galactosidase.

Physical form

Suspension in 0.1 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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X G Chen et al.
European journal of biochemistry, 221(2), 655-664 (1994-04-15)
Sialidase activities of rabbit blood cells and serum were measured. The leucocyte particulate fraction showed the highest specific activity of sialidase towards mixed gangliosides and sialyllactose, and the cytosolic fraction showed for fetuin. Predominant sialidase activity in the blood was
T Kiyohara et al.
Journal of biochemistry, 80(1), 9-17 (1976-07-01)
Three beta-N-acetylhexosaminidases [EC 3.2.1.52] and one beta-galactosidase [EC 3.2.1.23] were purified from the culture filtrate of streptococcus 6646 group K by a combination of column chromatographies on p-aminophenyl beta-D-thiogalactopyranoside-substituted Sepharose and N-(paminophenyl)oxamic acid-substituted Sepharose. These beta-N-acetylhexosaminidases showed optimal activities between
A Varki et al.
The Journal of biological chemistry, 258(20), 12465-12471 (1983-10-25)
The naturally occurring sialic acids can have different types of N- and O-substitutions, resulting in more than 20 known isomers and compounds. Most methods for the detailed study of these various sialic acids require that the molecules be first released

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