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Sigma-Aldrich

5(6)-Carboxytetramethylrhodamine

BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)

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About This Item

Fórmula empírica (notación de Hill):
C25H22N2O5
Número de CAS:
98181-63-6
Peso molecular:
430.45
MDL number:
MFCD16661194
UNSPSC Code:
12352116
PubChem Substance ID:
329752227
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥85% (sum of isomers, HPCE)

form

solid

mp

≥300 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 543 nm; λem 572 nm in methanol

suitability

suitable for fluorescence

SMILES string

CN(C)c1ccc2c(Oc3cc(ccc3C24OC(=O)c5cc(ccc45)C(O)=O)N(C)C)c1.CN(C)c6ccc7c(Oc8cc(ccc8C79OC(=O)c%10ccc(cc9%10)C(O)=O)N(C)C)c6

InChI

1S/2C25H22N2O5/c1-26(2)15-6-9-19-21(12-15)31-22-13-16(27(3)4)7-10-20(22)25(19)18-8-5-14(23(28)29)11-17(18)24(30)32-25;1-26(2)15-6-9-18-21(12-15)31-22-13-16(27(3)4)7-10-19(22)25(18)20-11-14(23(28)29)5-8-17(20)24(30)32-25/h2*5-13H,1-4H3,(H,28,29)

InChI key

LBUFYZITRTUEFI-UHFFFAOYSA-N

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Application

5(6)-Carboxytetramethylrhodamine (TAMRA, TMR) may be used as a fluorescence probe to study the distribution of molecules such as cell-penetrating peptides (CPPs) and as fluorescence partner in applications such as two-dimensional fluorescence intensity distribution analysis (2D-FIDA), luminescence resonance energy transfer (LRET) and fluorescence resonance energy transfer (FRET).
Reacts with primary and secondary amine groups on proteins, nucleic acids and other biomolecules.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

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Biosensors & bioelectronics, 43, 252-256 (2013-01-16)
In this short communication we report an efficient and versatile method for the detection of methicillin-resistant staphylococcus aureus (MRSA) DNA sequence with high sensitivity and specificity. This method is based on upconversion nanoparticles (UCNs) and luminescence resonance energy transfer (LRET)
Dongyun Liu et al.
The AAPS journal, 15(1), 288-298 (2012-12-01)
Peptide and protein drugs have become the new generation of therapeutics, yet most of them are only available as injections, and reports on oral local intestinal delivery of peptides and proteins are quite limited. The aim of this work was
P Kask et al.
Biophysical journal, 78(4), 1703-1713 (2000-03-29)
A method of sample analysis is presented which is based on fitting a joint distribution of photon count numbers. In experiments, fluorescence from a microscopic volume containing a fluctuating number of molecules is monitored by two detectors, using a confocal
Daniel Renčiuk et al.
Methods (San Diego, Calif.), 57(1), 122-128 (2012-04-03)
Some of the most serious diseases are characterized by the presence of a specific secondary structure within DNA or RNA, often in the promoter or the coding region of the responsible gene, that enhances or disrupts expression of the protein.
Yoshiaki Yano et al.
Biochemistry, 41(9), 3073-3080 (2002-02-28)
Investigation of interactions between hydrophobic model peptides and lipid bilayers is perhaps the only way to elucidate the principles of the folding and stability of membrane proteins (White, S. H., and Wimley, W. C. (1998) Biochim. Biophys. Acta 1367, 339-352).
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