Digoxigenin-11-UTP

Name: Digoxigenin-11-UTP
Brand: ROCHE

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NACRES:

NA.21

UNSPSC Code:

41105500

Assay:

≥85% (HPLC)

Form:

solution

Storage temp.:

−20°C

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Propiedades

assay

≥85% (HPLC)

form

solution

mol wt

(M_r= 1106.7 (DIG-11-UTP-Li4))

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

Descripción

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:

in situ hybridization
in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
fluorescence in situ hybridization (FISH)

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.