10127655001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

• Sinónimos: G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Comisión internacional de enzimas: 1.1.1.49 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: yeast
• Nivel de calidad: 100
• Formulario: suspension
• actividad específica: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
• envase: pkg of 1 mL (5 mg/ml)
• fabricante / nombre comercial: Roche
• concentración: ≥0.1-1.0 % (w/w)
• técnicas: activity assay: suitable
• color: white to yellowish
• pH óptimo: 9.2
• solubilidad: water: miscible
• idoneidad: suitable for UV spectrophotometry and general use
• Nº de acceso NCBI: CAA93357
• Nº de acceso UniProt: P11412
• aplicaciones: life science and biopharma
• actividad extraña: 6-PGDH <0.0100%; CK <0.00100%; GR <0.0100%; HK <0.0100%; PGI <0.00200%; PGluM <0.0100%
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: Saccharomyces cerevisiae ... ZWF1(855480)

Descripción

Descripción general

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

Aplicación

Component of cofactor recycling systems for NADPH.

Acciones bioquímicas o fisiológicas

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Especificaciones

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparación

Activator: Mg²⁺ (5 to 10 mM)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash