PC05
Anti-c-Fos (Ab-2) (4-17) Rabbit pAb
liquid, Calbiochem®
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
Acerca de este artículo
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
—
Citations:
42
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... FOS(2353)
General description
This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen sections, Immunoblotting, IF, IP, Paraffin sections, for the detection of c-Fos (Ab-2) (4-17).
Protein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.
Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
The proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
Immunogen
Human
a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
Application
Frozen sections (5-10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/reaction)
Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
Free-floating Sections (not recommended)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/reaction)
Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
Free-floating Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Positive Control
HepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
HepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
Other Notes
DeTogni, P., et al. 1988. Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
For gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)
Still not finding the right product?
Pruebe nuestra Herramienta de selección de productos para limitar sus opciones
Clase de almacenamiento
10 - Combustible liquids
wgk
nwg
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Contenido relacionado
A R Soderman et al.
Neuroscience, 154(4), 1506-1516 (2008-06-14)
Opioid receptor agonists and antagonists have profound effects on cocaine-induced hyperactivity and conditioned reward. Recently, the role specifically of the mu opioid receptor has been demonstrated based on the finding that i.c.v. administration of the selective mu opioid receptor antagonist
Natasha M Sosanya et al.
BMC neuroscience, 20(1), 17-17 (2019-04-25)
Reports show that stressful events before injury exacerbates post-injury pain. The mechanism underlying stress-induced heightened thermal pain is unclear. Here, we examined the effects of chronic intermittent stress (CIS) on nociceptive behaviors and brain-derived nerve growth factor (BDNF) system in
N Laflamme et al.
Journal of molecular neuroscience : MN, 8(3), 165-179 (1997-06-01)
The purpose of this study was to investigate the effect of the immune activator lipopolysaccharide (LPS) on the expression of corticotropin-releasing factor (CRF) and glucocorticoid receptor (GR) mRNA in the paraventricular nucleus (PVN) of transgenic mice with impaired GR function